二色补血草质量标准研究

Study on Quality Standard for Limonium Bicolor

目的对二色补血草的质量标准的建立进行研究。方法鉴别二色补血草的性状并且观察该药物的显微特征;采用TLC法进行定性鉴别并用HPLC法测定二色补血草中没食子酸的含量。结果对二色补血草的性状、显微特征进行了描述;通过多批次的测定结果,TLC具有斑点清晰和分离度良好;没食子酸在进样量为1. 251~7. 506μg(r=0. 9999)的范围内与峰面积呈现良好的线性关系,平均回收率为99. 86%,RSD为0. 66%(n=6)。结论建立的分析方法简便可行并且专属性强,可用于二色补血草的质量控制。

Objective： To study the establishment of quality standard for limonium bicolor. Method： the traits of limonium bicolor were identified and the microscopic characteristics were observed; qualitative identification were carried out by TLC method and determination of gallic acid in Limonium bicolor by HPLC. Result： The traits and microscopic characteristics of limonium bicolor were described. The results of multiple batches showed that TLC had clear spots and good resolution. Gallic acid showed a good linear relationship with peak area in the range of 1. 251-7. 506 UG（r = 0.9999）,with an average recovery of 99. 86% and RSD of 0. 66%（n = 6）. Conclusion： The analytical method established in this paper is simple,feasible and specific,and can be used for quality control of limonium bicolor.