基于60个核心SSR标记的糯玉米自交系遗传多样性分析

Genetic Diversity Analysis of Waxy Corn Inbred Lines Based on 60 Core SSR Markers

利用60个核心SSR标记分析了68份糯玉米自交系的遗传多样性与亲缘关系,为糯玉米育种材料的改良和利用提供参考依据。结果表明:60个核心SSR标记在68份糯玉米自交系中检测出414个等位变异,每个标记检测出的等位变异数为3～13个,平均为6.9个;基因多样性变幅为0.4982～0.9124,平均为0.7603; SSR标记多态性信息量(PIC)介于0.4630～0.8515之间,平均为0.7144;供试材料间的遗传距离变幅为0.1800～0.9600,平均为0.7402;利用UPGMA聚类分析法,将所有供试材料分为5大类群,分别为JS0381类群、衡白522类群、S181类群、通系5类群和中间类群;划分结果基本符合自交系的来源情况。68份糯玉米自交系的遗传多样性相对丰富,亲缘关系较远,作为糯玉米种质资源利用及品种选育具有一定的价值。

Sixty core SSR markers were used to explore the genetic diversity and genetic relationship of 68 waxy corn inbred lines, in order to provide reference for the improvement and application of waxy corn breeding materials. The results indicated that： the detected alleles of 60 core SSR markers in 68 waxy corn inbred lines were 414, and the detected alleles per maker ranged from 3 to 13, with an average of 6.9; the gene diversity ranged from 0.4982 to 0.9124, with an average of 0.7603; the polymorphic information content （PIC） of SSR markers was between 0.4630 and 0.8515, with an average of 0.7144; the genetic distance among the tested materials varied from 0.1800 to 0.9600, with an average of 0.7402; UPGMA clustering analysis divided the tested all materials into 5 groups （JS0381 group, Hengbai 522 group, S181 group, Tongxi 5 group, and uncertain group）, and this division result basically conformed to that based on geographical resources and germplasm genealogy. These 68 waxy corn inbred lines possess abundant genetic diversity and far genetic relationship, so they can be used for the breeding of waxy corn new varieties.