大高良姜、红豆蔻乙酸乙酯部位的体外抗氧化活性研究

Study of Da Gaoliangjiang and Hong Doukou ethyl acetate parts on Antioxidant Activity

目的：比较研究大高良姜、红豆蔻乙酸乙酯部位的体外抗氧化活性。方法：用无水乙醇将大高良姜、红豆蔻乙酸乙酯部位配制成不同质量浓度药液（5μg／ml，10μg／ml，40μg／ml，80μg／ml，100μg／ml，120μg／ml，160μg／ml），采用DPPH·自由基体系、ABTS＋·自由基体系、O2·自由基体系，结合酶标仪对不同质量浓度的大高良姜、红豆蔻乙酸乙酯部位的抗氧化活性进行比较研究。结果：大高良姜、红豆蔻乙酸乙酯部位均有体外抗氧化作用，且抗氧化活性大小顺序为：160μg／ml〉120μg／ml〉120μg／ml〉80μg／ml〉40μg／ml〉10μg／ml〉5μg／ml。结论：大高良姜、红豆蔻乙酸乙酯部位均具有体外抗氧化活性，且大高良姜乙酸乙酯部位清除ABTS＋及O2·自由基的能力强于红豆蔻乙酸乙酯部位，同时其抗氧化活性的大小均与其质量浓度有关系。

Objective： Comparative Study Da Gaoliangjiang ethyl acetate extract of Hong Doukou antioxidant activity. Methods： With anhydrous ethanol Da Gaoliangjiang and Hong Doukou formulated into various parts of ethyl acetate chemical concentrations （5μg/ml, 10μg/ml, 40μg/ml, 80μg/ml, 100 μg/ml, 120 μg/ml, 160 μg/ml, and using DPPH free radical system, ABTS^＋ · free radical system and O2^-· free radical system, Binding microplate reader galanga different concentrations of antioxidant activity of red cardamom ethyl acetate extract comparative study. Result： Da Gaoliangjiang and Hong Doukou of ethyl acetate parts are in vitro antioxidant effect, and the order of antioxidant activity is flowing ： 160μg/ml〉120 μg/ml〉100 μg/ml〉80 μg/ml〉40 μg/ml〉10μg/ml〉5μg/ml.Conclusions： Da Gaoliangjiang and Hong Doukou of ethyl acetate parts are in vitro antioxidant effect, And the ability of Da Gaoliangjiang to remove ABTS^＋ · and 02^-· ftee radicals was higher than that of the Hong Doukou ethyl acetate site, in addition while the size of its antioxidant activity and its mass concentration are related.