自愿咨询体检者血液标本进行乙型肝炎病毒血清学、核酸联合检测结果分析

Analysis of Hepatitis B Virus Serological and Nucleic Acid Combined Detection Results of Blood Samples Obtained from Voluntary Consultation

目的分析自愿咨询体检者血液标本进行乙型肝炎病毒血清学、核酸联合检测的结果。方法采集商丘市疾病预防控制中心感染科自愿咨询体检者的2 000份血液标本,使用两个不同类型厂家的ELISA试剂对乙肝表面抗原进行检测,两种试剂均阳性则使用单人份的NAT对HBV-DNA进行检测,当两种试剂表现为均阴性或是单试剂为阳性时,则实施6人份的混样检测,若是混样阳性则实施单人份的拆分检测,对两种检测方法的检出率是否符合进行比较。结果核酸检测以及血清学检测均阴性的共1 891份,核酸与血清学的检验均阳性共53份,血清学检验为阳性而核酸检验为阴性的43份,血清学检测为阴性而核酸检测为阳性的13份。HBsAg双试剂均为阳性的为93份,HBV-DNA为阳性的52份;HBsAg以及HBV-DNA符合率分别为55.91%、33.33%。结论在入职体检、健康体检和输血等有关传染病的检测中,血清学同核酸二者联合检测能够有效提升准确率,降低漏检率,任何一种检测方式的缺失都会导致血液漏检风险。

OBJECTIVE The results of HBV serology and nucleic acid detection were analyzed in the blood samples of the voluntary counselingand testing department of the infectious disease department. METHODS 2 000 blood samples were collected from the voluntarycounselling and inspector of the infectious family, and the ELISA reagents of 2 different types of manufacturers were used to detect the surfaceantigen of hepatitis B. The 2 reagents were tested using a single human NAT to test the HB-DNA. When the 2 reagents were negativeor the single reagent was positive, the 6 people were carried out. Mixed sample detection, if the mixture is positive, the single person splittest is carried out, and the detection rate of the 2 detection methods is compared. RESULTS Nucleic acid tests and serological tests were allnegative in 1891 copies; nucleic acid and serology were tested positive for a total of 53 specimens; serological tests were positive and nucleicacid tests were negative in 43; serological tests were negative and nucleic acid tests were positive for 13 copies. The double reagents ofHBsAg were all positive, 93 were positive, 52 were positive for HBV-DNA, and 55.91% and 33.33% for HBsAg and HBV-DNA respectively.CONCLUSION In the examination of medical examination, health examination, blood transfusion and other related infectious diseases,the combined detection of serology and nucleic acid two can effectively improve the detection accuracy, reduce the leakage rate, and thelack of any kind of detection method would lead to the risk of blood leak detection.