卵巢癌病灶内miR-451表达量与增殖、凋亡基因表达的相关性研究

Correlation of miR-451 expression with proliferation and apoptosis gene expression in ovarian cancer

目的:研究卵巢癌病灶内miR-451表达量与增殖、凋亡基因表达的相关性。方法:选择在我院接受手术切除的卵巢癌患者,收集卵巢癌病灶及癌旁病灶,分离miRNA并测定miR-451的表达量,分离mRNA并测定细胞周期蛋白、增殖基因、凋亡基因的mRNA表达量。结果:卵巢癌病灶内miR-451的表达量以及细胞周期蛋白激酶抑制因子1B(p27Kip1)、BCL2相关X蛋白(Bax)、自杀相关蛋白因子(Fas)、Fas配体(FasL)、半胱氨酸天冬氨酸蛋白酶-3(Caspase-3)、增强子结合蛋白同源蛋白(CHOP)的mRNA表达量均明显低于癌旁病灶,细胞周期蛋白B1(CyclinB1)、细胞周期蛋白D1(CyclinD1)、细胞周期蛋白E(CyclinE)、磷脂酰肌醇3激酶(PI3K)、蛋白激酶B(AKT)、β-连环蛋白(β-catenin)、基质金属蛋白酶2(MMP2)、基质金属蛋白酶9(MMP9)的mRNA表达量明显高于癌旁病灶;卵巢癌灶内miR-451与CyclinB1、CyclinD1、CyclinE、PI3K、AKT、β-catenin、MMP2、MMP9的mRNA表达量呈负相关,与p27Kip1、Bax、Fas、FasL、Caspase-3、CHOP的mRNA表达量呈正相关。结论:卵巢癌病灶内miR-451的低表达与细胞周期正性调控蛋白及增殖基因的高表达、细胞周期负性调控蛋白及凋亡基因的低表达密切相关,可能是促进卵巢癌病灶生长的关键因素。

Objective： To study the correlation of miR-451 expression with proliferation and apoptosis gene expression in ovarian cancer. Methods： The patients with ovarian cancer who underwent surgical resection in our hospital between March 2015 and February 2018 were selected, ovarian cancer lesions and adjacent lesions were collected, miRNA was isolated to determine the expression of miR-451, and mRNA was isolated to determine the mRNA expression of cyclin, proliferation genes and apoptosis genes. Results： The expression of miR-451 as well as the mRNA expression of Cyclin-dependent kinase inhibitor 1B （p27Kip1）, BCL2-associated X protein （Bax）, factor associated suicide （Fas）, Fas ligand （FasL）, cysteine aspartyl proteinase 3 （Caspase-3） and CCAAT/enhancer-binding protein homologous protein （CHOP） in ovarian cancer lesions was significantly （ P 〈0.05） lower than those in adjacent lesions while the mRNA expression of CyclinB1, CyclinD1, CyclinE, phosphatidylinositol 3-kinase （PI3K）, protein kinase B （AKT）, β-catenin, matrix metalloproteinase 2 （MMP2） and matrix metalloproteinase 9 （MMP 9） were significantly higher than those in adjacent lesions （ P 〈0.05）; miR-451 in ovarian cancer was negatively correlated with CyclinB1, CyclinD1, CyclinE, PI3K, AKT, β-catenin, MMP2 and MMP9 mRNA expression, and positively correlated with p27Kip1, Bax, Fas, FasL, Caspase-3 and CHOP mRNA expression. Conclusion： The low expression of miR-451 in ovarian cancer is closely correlated with the high expression of positive cell cycle regulatory proteins and proliferation genes as well as the low expression of negative cell cycle regulatory proteins and apoptosis genes, and it may be the key factor to promote the growth of ovarian cancer.