摘要
Polyurethane based on polypropylene glycol(PPG) and TDI using 3,3\|dichloride\|4,4\|methylenedianiline(MOCA) as the crosslinker is presented for the first time to fabricate molecular stamps(PU stamps) for the synthesis of DNA microarray with contact procedure. The predictability of the process is achieved by utilizing commercially available starting materials. SEM analysis of the morphology of PU stamps and photoresist motherboard showed that PU elastomer could replicate subtly the motherboards patterns with a high fidelity. It was proved from the contact angle measurement that PU stamps surface has a good affinity with acetonitrile, which guarantee the well\|distribution of DNA monomers on patterned stamps. Laser confocal fluorescence microscopy images of oligonucleotide arrays confirm that polyurethane is an excellent material for molecular stamps.
Polyurethane based on polypropylene glycol(PPG) and TDI using 3,3\|dichloride\|4,4\|methylenedianiline(MOCA) as the crosslinker is presented for the first time to fabricate molecular stamps(PU stamps) for the synthesis of DNA microarray with contact procedure. The predictability of the process is achieved by utilizing commercially available starting materials. SEM analysis of the morphology of PU stamps and photoresist motherboard showed that PU elastomer could replicate subtly the motherboards patterns with a high fidelity. It was proved from the contact angle measurement that PU stamps surface has a good affinity with acetonitrile, which guarantee the well\|distribution of DNA monomers on patterned stamps. Laser confocal fluorescence microscopy images of oligonucleotide arrays confirm that polyurethane is an excellent material for molecular stamps.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2002年第9期1823-1825,共3页
Chemical Journal of Chinese Universities
基金
国家八六三计划 (批准号 :10 3 -13 -0 5 -0 110 1-0 1-0 1)
九七三计划 (批准号 :G19980 5 12 0 4)
国家自然科学基金 (批准号 :60 0 710 0 1)
教育部"高等学校骨干教师资助计划"项目资助
