高效液相色谱法测定化妆品中辛酰羟肟酸的含量

Determination of octyl hydroxamic acid in cosmetics by high performance liquid chromatography

建立了高效液相色谱法测定化妆品中辛酰羟肟酸含量的分析方法。采用ZORBAX Eclipse Plus C_(18)柱(4.6mm×250mm,5μm),流动相为水-乙腈,流速1.0mL/min,柱温25℃,检测波长220nm,进样量10μL。结果表明,辛酰羟肟酸在10～200mg/L范围内,峰面积与质量浓度呈良好的线性关系,相关系数为0.9999(n=5),方法检出限为5mg/kg。高、中、低不同水平的加标回收率为96.28%～101.23%,相对标准偏差≤2.43%。该方法操作简便、精密度高、回收率高、重现性好,可用于化妆品中辛酰羟肟酸的检测。

A method for the determination of octyl hydroxamic acid in cosmetics by high performance liquid chromatography(HPLC) was established. ZORBAX Eclipse Plus C18 column(4.6 mm×250 mm, 5 μm) was used. The mobile phase was water and acetonitrile, the flow rate was 1.0 mL/min, the column temperature was 25 ℃, the detection wavelength was 220 nm and the injection volume was 10 μL. The results show that the peak area has a good linear relationship with the mass concentration of octanoyl hydroxamic acid in the range of 10-200 mg/L, and the correlation coefficient is 0.999 9(n=5). The limit of detection is 5 mg/kg. The recoveries of octyl hydroxamic acid at different spiked levels are 96.28%-101.23% with the relative standard deviations(RSD) less than 2.43%. This method is simple operation, high precision, high recovery and good reproducibility, and can be used to detect octyl hydroxamic acid in cosmetics.