山莨菪硷治疗再生障碍性贫血与体外试验的相关性研究

Correlation study of in vitro tests with clinical response to amisodamin therapy patients with aplastic anemia

用人骨髓细胞培养贴壁细胞层,建立基质层。在此层上培养粒系祖细胞(CFU-c),以不带基质层的CFU—c为对照(100％),发现第3周有基质层CFU-c产率为无基质层对照150.7±17.63％,此方法发现基质层上接种CFU-c,可反映再障病人骨髓微环境病变,基质层上生长CFU—c状况与山莨菪硷治疗呈负相关。本文为确定再障患者微环境病变建立了方法。

Fourteen patients were treated with ALG. Eight were detected co-culture of patient's lymphocytes with normal BM cell. in four patients the suppression of CFU-c was prevented by the pretreatment of the T lymphocyte with anti-T lymphocyte McAb and concordance with clicical outcome was observed in only two.Seven patients have received intravenous foetal liver cell suspension in addition to ALG therapy and remission was 'more complelete' in three.