钛阳极氧化载铜层的抗菌及生物相容性研究

Antibacterial Ability and Biocompatibility of Ti Anodized Copper Layer

在纯钛表面采用喷砂酸蚀、二次阳极氧化和电沉积载铜的方法制备载铜二氧化钛微纳米涂层,以研究其抗菌性及毒性。试件分为对照组:MNT(未载铜组),试验组:MNT-1(0.5 g/L载铜组),MNT-2(1.0 g/L载铜组),MNT-3(1.5 g/L载铜组)。钛片经前处理后在电压30 V下进行二次阳极氧化,通过电沉积方法将铜沉积在二氧化钛微纳米管结构上。应用场发射电子显微镜(FESEM)、能谱仪分析试件的表面形貌及构成元素;以牙龈卟啉单胞菌(Pg)检测其抗菌性能,用小鼠成骨细胞(MC3T3-E1)检测其细胞毒性及细胞相容性。结果表明:喷砂酸蚀的纯钛试件经阳极氧化后,表面有不同层次的凹坑状结构并伴有微纳米小管的形成,电沉积载铜后,试件表面均匀分布有单质铜颗粒;抗菌性随铜离子浓度的增加而增加;载铜涂层在早期能一定程度上促进MC3T3-E1细胞的增殖,但随着铜浓度及细胞培养时间的增加,呈现细胞毒性。0.5 g/L载铜涂层具有良好的抗菌活性,并能促进细胞增殖。

Copper-loaded titanium dioxide micro-nano coating on pure titanium surface was obtained by sandblasting and acid etching,secondary anodic oxidation and electrodeposited copper. The samples were divided as control group MNT( free copper-loaded) and experimental group MNT-1( 0.5 g/L copper loaded),MNT-2( 1.0 g/L copper loaded) and MNT-3( 1.5 g/L copper loaded). After pre-treatment,the titanium plate was subjected to secondary anodic oxidation at a voltage of 30 V,and then copper was loaded on the titanium dioxide micro-nanotube structure by electrodeposition. Additionally, field emission scanning electron microscope( FESEM) and energy dispersive spectrometer( EDS) were utilized to analyze the surface morphology and elements of the specimen. The antibacterial activity of Pg was tested,and the cytotoxicity and cell compatibility of the cells were detected by mouse osteoblasts( MC3T3-E1). Results showed that the pure Ti sample after sandblasting and etching pre-treatment was anodized,and some pit-like structures in different levels and micro-nano tubes formed on the surface. After the copper was electrodeposited on the surface of the sample,single-component copper particles were uniformly distributed on the surface. The antibacterial activity increased with the increase of copper ion concentration. The copper-loaded coating promoted the proliferation of MC3T3-E1 cells to a certain extent in the early stage,but the cytotoxicity appeared afterwards with the increase of copper concentration and cell culture time. When the copper-loaded content was 0.5 g/L,the as-obtained coating possessed good antibacterial activity and could promote cell proliferation.