肝素化金纳米粒子的制备及其对人抗凝血酶Ⅲ的检测

Preparation of Heparin-modified Gold Nanoparticles for Human AntithrombinⅢ Detection

本研究利用肝素和抗凝血酶Ⅲ(AT-Ⅲ)可特异性结合的性质,将肝素固定在金纳米粒子表面,用于抗凝血酶Ⅲ的检测。制备了四种具有不同大小的肝素化金纳米粒子(Hep-AuNPs),透射电子显微镜观测结果表明其分散性良好,平均粒径在修饰前后无明显变化,分别为15nm、42nm、66nm和大于100nm。热失重分析结果表明四种Hep-AuNPs表面肝素结合量分别为24.95%、23.90%、22.64%和19.32%。通过对比不同Hep-AuNPs对AT-Ⅲ的测试结果,发现在1~26μmol/L浓度范围内,平均粒径为15nm的Hep-AuNPs在531nm处的吸光值变化与AT-Ⅲ浓度呈良好线性关系,相关系数(R2)为0.996,检出限(3σ)为0.003μmol/L,检测重复性好,有望用于临床AT-Ⅲ检测。

Heparin-modified gold nanoparticles(Hep-AuNPs)were prepared by immobilizing heparin on the surface of AuNPs.Based on the specific recognition between heparin and AT-Ⅲ,they could be used for antithrombinⅢ(AT-Ⅲ)detection.Four kinds of Hep-AuNPs of different average size were prepared,and TEM observation showed their average size were 15 nm,41nm,66 nm and>100nm,respectively,with well-dispersed nature.Meanwhile,TGA results showed that the immobilized amount of heparin were 24.95%,23.90%,22.64%and 19.32% on each kind of Hep-AuNPs.By comparing the detection results,it was found that the UV-vis absorption of Hep-AuNPs(15nm)at531nm had optimal linearity against the concentration of AT-Ⅲranging from 1to 26μmol/L.The detection limit is0.003μmol/L(3σ),the coefficient correlation(R2)is 0.996 and the method is repeatable.This novel Hep-AuNPs is promising to be used for clinical AT-Ⅲdetection.