大鼠维生素E营养状况与TNT急性染毒后肝脏巯基和钙稳态的关系

A Study on Relationship between Vitamin E Nutritional Status and Liver Thiol and Calcium Homeostasis after TNT Acute Administration in Rats

分别给摄食普通饲料和维生素E缺乏饲料40天的两组雄性Wistar大鼠用TNT(1000mg/kg)花生油溶液灌胃染毒,观察8小时和16小时后肝匀浆GSH、磷酸化酶a、线粒体和微粒体蛋白巯基及^(45)Ca摄取的变化。结果表明,TNT急性染毒对肝巯基和钙稳态的影响取决于维生素E的营养状况。食用普通饲料的动物在染毒后8小时除线粒体^(45)Ca摄取和肝匀浆GSH有明显下降外,其余指标均只有轻微变化,染毒后16小时各项指标均恢复至接近对照组水平。但在食用维生素E缺乏饲料的动物,上述变化更为显著,且随着染毒时间的延长至16小时,不仅未见恢复趋势,而且继续加剧。

Both groups of male Wistar rats fed with common and vitamin E deficientdiets were administrated by oral gavage with TNT(1000mg/kg) in peanutoil,respectively,and decapitated 8 or 16 hrs later.The indices including GSHand phosphorylase a in liver homogenates,protein-SH and ^(45)Ca uptake ofmitochondria and microsomes were immediately assayed.It was shown thatthe effects of TNT on liver thiol and calcium homeostasis were dependent onvitamin E nutritional status.In the common diet group,most indices were onlyslightly changed except for obvious inhibition of mitochondrial ^(45)Ca uptakeand reduction of liver homogenate GSH,8 hr after TNT administration,whichwere rapidly recovered to about control levels in 16 hr.However,theafore-mentioned perturbation,were markedly aggravated and developed,asthe time of TNT administration increased to 16 hrs in the Vitamin E deficientgroup.