马铃薯茎尖组织培养及快速繁殖技术研究

Tissue culture and rapid propagation of Solanum tuberosum L.shoot tip

以豫马铃薯 1号 (郑薯 5号 )和津引 8号马铃薯为外植体 ,应用不同的培养基对马铃薯茎尖进行组织培养 ,以所获得的无毒苗为材料进行快速繁殖研究 .结果表明 ,豫马铃薯 1号外植体在MS +BA 2mg·L-1+NAA 0 .1mg·L-1+GA30 .1mg·L-1+蛋白胨 10 0mg·L-1培养基上成苗率较高 ;津引 8号马铃薯在MS +BA 1.5mg·L-1+NAA 0 .1mg·L-1+GA30 .1mg·-1+蛋白胨 10 0mg·L-1培养基上成苗率较高 ;而 1/ 2MS +IBA 0 .3mg·L-1,1/ 2MS +IAA 0 .5mg·L-1培养基适宜于豫马铃薯 1号无毒苗的快速繁殖 ;1/ 2MS +IBA 0 .3mg·L-1,1/ 2MS +IAA 0 .3mg·L-1培养基适宜于津引 8号无毒苗的快速繁殖 .

The experiments were conducted on the “Zhengshu NO.5”and“Jinyin NO.8”.The apical meristems of explants were cultured on the media with different hormones.The results showed that the explant of “Zhangshu NO.5” mutiplicated rapidly on the MS+BA 2 mg·L -1+NAA 0.1 mg·L -1 +GA 3 0.1 mg/lL medium, The explant of “Jinyin NO.8” can propagate rapidly on the MS+BA 1.5 mg·L -1 +NAA 0.1 mg·L -1+GA 3 0.1 mg·L -1 medium.“Zhengshu NO.5”on the MS+BA 1.5 mg·L -1 and MS+IAA 0.3mg·L -1 medium, “Jinyin NO.8”on the MS+IBA 0.3 mg·L -1 and MS+IAA 0.5 mg·L -1 medium can obtain good results.