尾悬吊模拟失重大鼠肺组织一氧化氮合酶的变化

Changes in nitric oxide synthase in pulmonary tissues during tailsuspension simulating weightlessness in rats

目的 :研究尾悬吊模拟失重大鼠肺组织一氧化氮合酶 (NOS)的变化。方法 :采用尾悬吊模拟失重 ,分为悬吊 7日和 2 1日及相应对照 4个组 ,每组 10只健康雄性 SD大鼠 ,共 4 0只大鼠。并采用免疫组织化学技术检测肺组织 NOS的表达情况。结果 :同正常对照组相比 ,7日尾悬吊模拟失重大鼠肺组织诱导型一氧化氮合酶(i NOS)的表达水平明显增高 (P<0 .0 5 ) ;2 1日的表达水平仍显著增高 (P<0 .0 5 ) ,同时可见血管内皮细胞随着时间延长阳性数目显著增多 (P<0 .0 1)。结论 :在模拟失重条件下肺组织 i

Objective:To investigate the changes in nitric oxide synthase in pulmonary tissues of rats during tailsuspension simulating weightlessness.Methods:Experiments were conducted on 40 male Sprague Dawley rats,and they were divided into 4 groups with 10 rats in each:suspended for 7 days,control of 7 days and suspended for 21 days,control of 21 days.Tailsuspension(30°) was used to simulate weightlessness.The activity of nitric oxide synthase in pulmonary tissues was examined by immunohistochemistry.Results:The level of inducible nitric oxide synthase in pulmonary tissues of both 7 days and 21 days tailsuspended rats increased significantly( P <0 05 for both groups).Conclusions:The level of inducible nitric oxide synthase increases during simulated weightlessness.