血管紧张素转换酶基因多态性与高血压腔隙性脑梗死关联性的研究

A Study of the Relationship Hetween Angiotensin - converting Enzyme Gene Nolymorphism and Lacunar In- farction in Patients with Essential Hypertension

目的 研究血管紧张素转换酶(ACE)基因插入/缺失/(I/D)多态性与高血压腔隙性脑梗死的关系。方法(1)应用聚合酶链反应(PCR)方法扩增50例正常人、49例高血压无并发症患者、30例高血压腔隙性脑梗死患者的ACE基因上287 Bp片段,根据插入(I)或/缺失(D)来判断其多态性。(2)用CT或MRI诊断腔隙性脑梗死。结果 (1)腔隙性脑梗死组与健康对照组相比,其D等位基因及DD基因型显著升高。微量蛋白尿组与健康对照组相比,其D等位基因及DD基因型显著升高。(2)腔隙性脑梗死组与高血压无并发症组相比,其D等位基因及DD基因型显著升高。(3)高血压无并发症组与健康对照组相比,ACE基因型和等位基因频率无显著性差异。结论 ACE基因多态性与高血压腔隙性脑梗死患者有关联性,DD基因型提示可能与高血压腔隙性脑梗死有关。

To investigate the relationship between angiotensin- converting enzyme(ACE) gene polymorphism and lacunar infarction in patients with essential hypertension. Methods (1) Polymerase chain reaction (PCR) was used to determine the genotypes for an insertion/deletion polymorphism of the ACE gene in 49 hypertension patients and 30 hypertension patients with lacunar infarction and 50 healthy controls. (2) Lacunar infarction were diagnosed by MRI of CT. Results There was significant difference between the frequencies of D allele and DD genotype in hypertension patients with lacunar infarction and healthy controls. There was significant difference between the frequencies of D allele and DD genotype in hypertensive patients with lacunar infarction and non - lacunar infarction. There was not difference between ACE I/D polymorphism in hypertension patients with non- lacunar infarction and healthy controls. Conclusions There was correlation between ACE gene polymorphism and the hypertension with lacunar infarction. DD genotype was possibly involved in the lacunar infarction in the patients with hypertension.