肠炎沙门氏菌接种对雏鸡免疫器官指数的影响

The Effect of Salmonella Enteritidis Inoculation on Immune Organ Index of Jining Bairi Chicken

为探讨家禽对肠炎沙门氏菌感染的遗传免疫效应,本研究选用济宁百日鸡阴性个体为试验材料,用肠炎沙门氏菌进行接种,分别在接种后第1、3、7、14和21天称重屠宰,采集脾脏,胸腺和法氏囊等组织样品并称重。试验结果显示,肠炎沙门氏菌感染能抑制鸡体重的增加,但不显著。而对脾脏的影响主要体现在接种菌后第7天,其器官指数在肠炎沙门氏菌接种后降低0.04%;接种后第21天,试验组法氏囊指数比对照组显著增加;胸腺在接种后第21天前,细菌对其影响较小,但在接种后第21天,器官指数显著降低。本研究结果初步揭示了肠炎沙门氏菌感染对雏鸡体重和免疫器官的影响,这些作用因接种后的不同时间而有差异,为今后肠炎沙门氏菌感染家禽的机制研究提供了理论依据。

To explore the genetic immune response to Salmonella enteritidis (SE) infection of poultry, two-day old SE negative Jining Bairi chicken were selected as the experimental material and inoculated with SE. The chickens were slaughtered at 1、3、7、14 and 21 days after inoculation. The spleen, thymus and bursa of fabricius samples were collected and weighed. The results showed that the infection of SE did not influence chicken weight gain significantly, and the organ index of spleen was significantly decreased by 0.04% on 7 days after inoculation. The organ index of Bursa in tested group was significantly increased compared with the control group on 21 days after inoculation. Before 21 days after inoculation, the bacteria on the thymus had relatively little effect, but organ index of thymus was significantly decreased at 21 days after inoculation. This study preliminarily reveals that the body weight and immune organ index were affected by SE inoculation, and this effect was distinct at different time-points after SE inoculation. The results in this study paved the theoretical foundation of the mechanism of SE infection in poultry.