镁合金表面飞秒激光制备促骨细胞生长显微结构

Femtosecond Lasers Induced Micropatterns on Magnesium Alloy to Promote Cell Proliferation

采用飞秒激光在AZ31B镁合金表面制备了利于成骨细胞生长的显微图案化结构。研究表明,飞秒激光处理后,镁合金表面形成了由条纹、孔洞、微米颗粒以及覆盖整个表面的纳米颗粒组成的多级粗糙结构,且其亲水性发生显著改变,当激光通量为0.64 J/cm2时,样品表面润湿角降低至15°,且表面生成较多的MgAl2O4及Al2O3等耐腐蚀相,在一定程度上提高了样品的表面腐蚀电位。体外成骨细胞培养表明,在以通量为0.64 J/cm2的飞秒激光处理样品表面,MC3T3-E1细胞具有最快的生长速度,表明飞秒激光处理获得的显微图案有利于成骨细胞的攀附、增殖。

Femtosecond lasers were used to prepare micropatterns on AZ31 B magnesium alloy. The study shows that hierarchical roughness structures formed by strips, holes, micro-particles and nanoparticles are generated on the surface after femtosecond laser treatments. The contact angle is decreased to 15? after the femtosecond laser treatment with a fluence of 0.64 J/cm2. MgAl2O4 and Al2O3 were produced during the femtosecond laser processing, which increases the corrosion potentials of the alloys. In vitro cell culture test indicates that MC3T3-E1 osteoblastic cells grow faster on the samples treated by femtosecond lasers, especially with the laser fluence of 0.64 J/cm2, suggesting that micropatterns formed by femtosecond lasers are favorable for osteoblastic cell proliferation.