溶胶凝胶壳聚糖膜促进纺锤形赖氨酸芽孢杆菌转化异丁香酚生成香草醛的研究（英文）

Promotion of Biotransformation from Isoeugenol to Vanillin by Lysinibacillus Fusiformis with Sol-gel Chitosan Membrane

通过溶胶凝胶冷冻干燥法制备的壳聚糖膜用于结合纺锤形赖氨酸芽孢杆菌CGMCC1347生物转化异丁香酚中的产物香草醛。壳聚糖与香草醛能形成希夫碱。本研究优化了其结合条件及洗脱条件。研究结果表明,在pH7.0、37℃、200r/min、48h,1g壳聚糖可结合0.125g香草醛。壳聚糖膜上所结合的香草醛以6.17%(w/w)HCl溶液室温下放置10h可完全洗脱。在此生物转化过程中添加壳聚糖膜可有效避免产物抑制。优化后的转化条件为装液量10mL/50mL锥形瓶、异丁香酚2%(v/v)、湿细胞0.48g、壳聚糖膜0.1g、磷酸缓冲液(pH8.0)50mmol/L、37℃、180r/min转化60h,香草醛最高浓度达1.71g/L。

The chitosan membrane was prepared by a sol-gel freeze-drying method and used to remove the produced vanillin in time from the biotransformation system of isoeugenol to vanillin by Lysinibacillus fusiformis CGMCC1347. The chitosan and vanillin could be linked by the formed schiff bases. The combining and eluting conditions were optimized. As a result, 1 g chitosan membrane could combine 0.125 g vanillin under the condition of p H 7.0, 37 oC, and 200 r/min in 48 h. All the combined vanillin could be eluted from the chitosan membrane with 6.17%(w/w) aqueous HCl at room temperature in 10 h. The product inhibition could be avoided by the addition of chitosan membrane during the biotransformation. The optimal biotransformation conditions are 2%(v/v) isoeugenol, 0.48 g wet cells, 0.1 g chitosan membrane, 50 mmol/L phosphate buffer(p H 8.0), 37 oC, 180 r/min for 60 h in 10 m L reaction liquid in 50 m L flask and the highest vanillin concentration reaches 1.71 g/L.