电针足三里对大鼠氧自由基和前列腺素的影响

Effects of Electroacupuncture at Zusanli on Oxygen Free Radicals and Plasma Prostaglandin in Stress Rats

目的 观察电针对应激大鼠血浆超氧化物歧化酶 ( SOD)、丙二醛 ( MDA)和前列腺素 E2 ( PGE2 )的影响 ,以探讨电针对胃黏膜保护作用的机理。方法 将 72只大鼠平均分为空白对照组、应激组和电针组 ,每组再按实验时间 1 ,3,5 d平均分为 3小组 (每小组 8只 )。利用生化比色法和放免分析法测定各组大鼠血浆 SOD、MDA和 PGE2 含量并计算各组溃疡指数 ( UI)。结果 应激组较空白对照组大鼠血浆 SOD明显下降 ( 1 7.0± 2 .9) N.L-1→ ( 1 1 .9± 3.4) N.L-1;MDA明显上升 ( 4 .85± 2 .38) N.L-1→ ( 7.5 6± 2 .48)N.L-1;PGE2 明显下降 ( 2 .74± 0 .77) ng.L-1→ ( 1 .5 4± 0 .2 5 ) ng.L-1,P<0 .0 5。 UI明显上升 ( 1 .1± 0 .4)→ ( 2 8.0± 4.1 ) ,P<0 .0 1。电针组较应激组血浆 SOD明显上升 ( 1 1 .9± 3.4) N.L-1→ ( 1 7.7± 4.8) N.L-1;MDA明显下降 ( 7.5 6± 2 .48) N.L-1→ ( 4 .1 4± 1 .78) N.L-1;PGE2 明显上升 ( 1 .5 4± 0 .2 5 ) ng.L-1→( 3.2 1± 0 .38) ng.L-1,UI明显下降 ( 2 8.0± 4.1 )→ ( 1 9.0± 2 .3) ;P<0 .0 1。电针组 PGE2 实验 5 d组较实验 1 d组明显上升 ( 3.2 1± 0 .38) ng.L-1→ ( 4 .5 2± 1 .0 6) ng.L-1,P<0 .0 5。结论 电针对胃黏膜的损伤具有保护作用 ,其机制与其影?

Objective To stuby the effect of electroacupuncture(EA) on plasma superoxide dismutase (SOD),malondialdehyde(MDA) and prostaglandin E 2(PGE 2) in stress rats.Metnods 72 SD rats were randomly divided into 3groups:control group,stress group and EA group;each group were divided into 3small groups by 1,3,5 d test time.The plasma levels of SOD and MDA were measured by biochemical methods,the levels of PGE 2 by radioimmunosassay methods.The extent of gastric mucosa damage was evaluated by ulcer index (UI),according to the truthful exponential method.Results In the stress group,the plasma levels of SOD decreased (17 0±2 9)N.L -1 →(11 9±3 4)N.L -1 ;MDA increased(4 85±2 38)N.L -1 →(7 56±2 48)N.L -1 ;PGE 2 decreased(2 74±0 77) ng.L -1 →(1 54±0 25 ng.L -1 , P >0 05;UI increased (1 1±0 4)→(28 0±4 1), P <0 01,as compared with those of control group.In the EA group compared wiht stress group,the plasma levels of SOD increased (11 9±3 4)N.L -1 →(17 7±4 8)N.L -1 ;MDA decreased (7 56±2 48)N.L -1 →(4 14±1 78)N.L -1 ;PGE 2 increased (1 54±0 25)ng.L -1 →(3 21±0 38) ng.L -1 ;UI decreased(28 0±4 1)→(19 0±2 3), P <0 01?The changes of plasma levels of PGE 2 in 5d test was more obvious than those in 1d in EA group (3 21±0 38)ng.L -1 →(4 52±1 06)ng.L -1 , P <0 05.Conclusion EA can protect gastric mucosa in stress rats.This function may be connected with its effection on oxygen free radicals and prostaglandin PGE 2 in EA group is effected by test time.