胃癌淋巴结微转移凝集素受体检测的意义

Lectin receptor detection of lymph node micrometastases in gastric carcinoma

目的 探讨凝集素受体的检测与胃癌淋巴结微转移的关系及意义。方法 应用亲合组织化学ABC法(avidinbiotionperoxidasecomplexABC) ,对 5 5例胃癌的 376个淋巴结进行生物素化凝集素 (Biotion -PHA)的标记。结果 HE染色检测 86个淋巴结有肿瘤转移 ,且PHA标记亦阳性。但另外检测出 39个淋巴结有微转移。进展期胃癌淋巴结微转移的阳性率明显高于早期胃癌 (P <0 .0 5 ) ,且弥漫型胃癌中淋巴结微转移高于肠型胃癌 (P <0 .0 5 )。结论 应用PHA作为探针来检查胃癌的淋巴结微转移 ,其敏感性高 ,特异性强 ,并有助于判断预后和指导治疗。

Objective To study the relationship between the lectin receptor detection and lymph node micrometastases in gastric carcinoma.Methods Biotin PHA were used to study 376 lymph nodes of 55 cases of gastric carcinoma by affinity histochemical method.Results 86 lymph nodes were positive both on HE and PHA. There were 39 HE negative lymph nodes that found micrometastases by affinity histochemical method. Micrometastases in advanced gastric carcinoma was markedly higher than that in early gastric carcinoma (p<0.05); Micrometastases showed a positive correlation with Lauren type.Conclusions Detection of micrometastases of lymph nodes by using PHA as biological marker is sensitive and specific for gastric carcinoma ,and it might be useful in evaluating patient's prognosis and treatment in gastric carcinoma.