猪链球菌2型的PCR快速检测

Rapid Detection of Streptococcus suis Type 2 by PCR

根据猪链球菌 2型的荚膜多糖抗原基因 cps2 J,合成 1对可扩增长度为 6 75 bp目的片段的引物 ,建立了检测猪链球菌 2型的 PCR法。应用 PCR对 9株经玻片凝集试验检测为猪链球菌 2型的菌株进行了检测 ,均呈阳性 ;而对马链球菌兽疫亚种 (C群 )、猪葡萄球菌、猪丹毒杆菌、猪肺疫巴氏杆菌、猪肺炎霉形体等检测结果均呈阴性 ,表明了本方法的特异性。用此法对 88份正常猪的扁桃体样品的细菌分离物进行了检测 ,36份呈阳性 ,同时用玻片凝集试验进行对照检测 ,也全部呈阳性。而此法不需进行细菌的纯分离培养 ,即可用于猪链球菌 2型的快速诊断以及流行病学调查。

A PCR for rapid and specific detection of Streptococcus suis type 2 was developed. The primers based on the cps2J-gene of S.suis type 2 could extend a 675 bp PCR product. Of 9 strains of S.suis type 2 isolated from diseased pigs, all were positive by the PCR. Using the same pairs, no PCR product was detected from S.equi subsp zooepidemicus, Staphylococcus hyicus, E.rhusiopathiae, P.pneumotropica and M.hyopneumoniae. Of 88 porcine tonsillar specimens, 36 was positive by the PCR or glass agglutination test. The corresponding rate was 100%. The results demonstrated that the PCR is a highly specific and rapid diagnostic tool for detection of S.suis type 2 and can be used to fast diagnosis and epidemic survey.