摘要
利用PCR技术 ,以绿脓杆菌羊分离株 (暂命名为PA_SD_1株 )的染色体DNA为模板 ,成功扩增出了该菌的主要致病基因 (绿脓杆菌外毒素A)Ⅲ区基因。将该PCR扩增片段与pGEMR_TEasy载体连接 ,获得了含有目的片段的重组质粒 (命名为PA_SD_ETA)。序列测定结果表明。该菌株与其他已报道的绿脓杆菌菌株有较高的同源性 ,而局部出现单个碱基的点突变。
Using polymerase chain reaction technique,DomainⅢ gene of Exotoxin A was amplified from the chromosomal DNA of one field isolates of sheep pseudomonas aeruginosa.the PCR amplified gene was linked with pGEM R-T Easy Vector,then transformed into E.coli strain DH5α,the recombinant plasmid contained 0.658kb fragment was obtained.Sequece analysis showed that the isolate from sheep shared high identity with the other isolates from human.
出处
《中国预防兽医学报》
CAS
CSCD
北大核心
2002年第5期338-340,共3页
Chinese Journal of Preventive Veterinary Medicine