各旋毛虫隔离种某些生物学特性研究

Studies on Some Biological Features of Trichinella Isolates

通过对猪、犬旋毛虫和国际标准隔离种 :旋毛形线虫 (Trichinellaspiralis)和本地毛形线虫 (Trichinellanativa)的研究发现 ,猪旋毛虫和旋毛形线虫在小鼠隔肌中出现保姆细胞的时间比较早 ,分别于感染第 16天和 18天出现 ,第 38天和 36天所有幼虫都已形成保姆细胞 ,而犬旋毛虫和本地毛形线虫出现保姆细胞的时间较晚 ,于感染第 2 0天和 2 2天出现 ,第 32天完全形成。猪旋毛虫和旋毛形线虫雌虫体外培养 2 4小时平均产新生幼虫数分别为 6 6 0和 76 2 ,而犬旋毛虫和本地毛形线虫分别是2 8.8和 2 2 .0 ,前二者在雌虫体外产新生幼虫能力上明显高于后二者。研究结果表明 ,黑龙江猪旋毛虫为旋毛形线虫 。

Some biological features of Trichinella from swine and dog in Heilongjiang province,China were firstly studied,the international standard Trichinella isolates,T.spiralis and T.nativa were used as the control.The experimental results indicated that development time of nurse cells around muscle larvae in mice diaphragms for 4 Trichinella isolates was different,the earliest muscle nurse cell development(encapsulation)date were at 16 days postinfection(d.p.i.)for swine isolate,at 18 d.p.i. for T.spiralis,at 20 d.p.i. for dog isolate and 22d.p.i. for T.nativa.The interval at which 100% of the muscle larvae became encapsulated was not related to the time of appearance of the first encapsulated larvae,the 100% level of encapsulation occurred at 32 d.p.i. for dog isolate and T.nativa,at 36 d.p.i. for T.spiralis and at 38 d.p.i. for swine isolate.In vitro,swine isolate and T.spiralis were more productive,with an average of total production of 55.00±7.34 and 76.2±7.57 newborn larvae(NBL) released per female worm in 24 hours of cultivation respectively,dog isolate and T.nativa had lower production,with an average of 28.8±4.30 and 22.00±3.22 NBL/female,respectively.On the basis of those results,we concluded that Trichinella from swine and dog in Heilongjiang province, China belong to Trichinella spiralis and Trichinella nativa,respectively.