嗜肾型鸡传染性支气管炎病毒W93疫苗株S1基因的克隆与序列测定

Sequencing and Homology Analysis of the S1 Gene of Nephropathogenic Strain W93

根据国外已发表的鸡传染性支气管炎病毒 (IBV)S1基因序列 ,设计了两对引物并以RT_PCR特异性扩增出嗜肾型IBVW93疫苗株的S1基因 ,基因产物大小为 1.6 2kb ,与设计相符 ,对其进行序列测定后 ,与标准毒株H5 2、H12 0、M41、BEAU株的S1基因进行同源性比较 ,结果表明 ,W93株与H5 2、H12 0、M41和BEAU株的核苷酸序列的同源性分别为 96 .8%、97.1%、96 .9%和 96 .8% 。

One pair of primers for amplifying the S1 gene of avian infectious bronchitis virus (IBF) had been designed,which the predicted 1.62kb fragment had been amplified via RT-PCR from Nephropathogenic Strain W93.The S1 gene of Nephropathogenic Strain W93 was sequenced and compared with standard strains such as H52,M41,H120 and BEAU.The results showed that the homology of nucleic acids between W93 strain and H52,M41,H120 and BEAU strains was 96.8%,97.1%,96.9% and 96.8% respectively.The high homology of the S1 gene of Nephropathogenic Strain W93 with those of other IBV standard strains had been proved.