一株类4/91病毒的初步研究

Studies on the Isolated Strain of IBV which was Similar to 4/91

从发病鸡群中分离到一株病毒 ,通过鸡胚接种、电镜观察、动物回归试验、气管环交叉中和试验等 ,初步确定为传染性支气管炎病毒 ,暂命名为A2。外源病原检查结果为阴性。攻毒试验 :在气管、肾以及肌肉等组织均有明显的病变 ;病理组织观察 ;在气管、肾脏和肌肉均发生了一定程度的病理变化。利用电泳对传统的病毒株和分离株进行了病毒结构蛋白分析 ,A2株出现了 31kD的特殊蛋白条带。气管环交叉中和试验表明A2株与 4/ 91病毒为同一血清型 ;临床的动物免疫试验结果表明 ,4/

Isolated from a sick breed,a virus named A2 and proved to be infectious bronchitis virus(IBV) by injection of chicken embryos,observing by electronscope,animal regress test,tracheal ring across neutralize test.The result of the test of outer virus was negative.The chickens had distinct pathological changes on tracheas,kidneys and muscles after being challenged by the virus,and with histopathological changes either.Electrophoresis the isolate with standard strain,the result showed that A2 had 31kD special protein.Tracheal ring across neutralize test suggested that A2 and 4/91 were the same serotype,and the 4/91 vaccine could protect the chickens challenged by A2.