摘要
NORK and soybean acyl carrier protein(ACP) both play important roles in nodulation.However,the relationship between Nod factor signaling and fatty acid(FA) biosynthesis during symbiotic development is unknown.In this study,an RNAi plasmid of Gm NORK was constructed and transformed into soybean roots by Agrobacterium rhizogene-mediated hairy-root transformation.The nodule number decreased substantially in Gm NORK knockdown soybean transgenic roots.To investigate the relationship between Gm ACP and Nod factor signaling,we measured Gm ACP expression levels in Gm NORK RNAi soybean transgenic roots and found that rhizobia inoculation led to substantially reduced Gm ACP expression.Thus,FA biosynthesis was affected by Nod factor signaling during nodule development in soybean,a finding that provides valuable information that improves our understanding of the functions of Gm NORK and Gm ACP in symbiotic signaling and nodule development.
NORK and soybean acyl carrier protein(ACP) both play important roles in nodulation.However,the relationship between Nod factor signaling and fatty acid(FA) biosynthesis during symbiotic development is unknown.In this study,an RNAi plasmid of Gm NORK was constructed and transformed into soybean roots by Agrobacterium rhizogene-mediated hairy-root transformation.The nodule number decreased substantially in Gm NORK knockdown soybean transgenic roots.To investigate the relationship between Gm ACP and Nod factor signaling,we measured Gm ACP expression levels in Gm NORK RNAi soybean transgenic roots and found that rhizobia inoculation led to substantially reduced Gm ACP expression.Thus,FA biosynthesis was affected by Nod factor signaling during nodule development in soybean,a finding that provides valuable information that improves our understanding of the functions of Gm NORK and Gm ACP in symbiotic signaling and nodule development.
基金
supported by the PhD Early Development Program of Yangtze University (0121)
