牛胎儿成纤维细胞的分离与体外培养

DISSOCIATION AND IN VITRO CULTURE OF BOVINE FIBROBLASTS

分别用胶原酶Ⅲ和胰蛋白酶成功地分离了牛胎儿成纤维细胞,并对其进行了体外培养,同时探讨了两种酶消化分离牛胎儿组织的时间对牛胎儿成纤维细胞原代培养的影响。用组织块直接培养也成功得到了牛胎儿成纤维细胞,并探讨了不同组织块大小对牛胎儿成纤维细胞原代培养的影响。通过绘制生长曲线,可以研究牛胎儿成纤维细胞增殖规律;通过制备牛胎儿成纤维细胞的细胞染色体发现,经过传代(12代)、冷冻保存、解冻,牛胎儿成纤维细胞染色体数目不变。

The bovine fetal fibroblasts were successfully dissociated and cultured in vitro .by using trypisn and collagenase Ⅲ . It was indicated that ihe digestion time of two kinds of enzyme had great influence on the primary culture of the bovine fetal fibroblasts. The bovine fetal fibroblasts could also be obtained by tissue block culture,and the size of tissue block had influence on the primary culture of the bovine fetal fibroblasts. Through analyzing the growth curve, the proliferation - promoting law of the bovine fetal fibroblasts could be studied; after the cell chromosomes were prepared, it was found that there were no changes in the chromosome number of the bovine fetal fibroblasts, which were subcultured for 12 passages,cryo-preserved and thawed.