β-榄香烯乳剂逆转多药耐药细胞株MCF-7/ADM对阿霉素耐药性研究

REVERSAL EFFECT OF β-ELEMENE ON MULTIDRUG RESISTANCE IN HUMAN TUMOR CELLS MCF-7/ADM

目的 :测定中药 β-榄香烯乳剂对 MCF- 7/ ADM细胞的无毒剂量 ,并检测此无毒剂量是否有逆转 MCF- 7/ ADM细胞对化疗药物阿霉素 (ADM)的多药耐药 (multidrug resistance,MDR)性。方法 :采用四甲基偶氮唑蓝 (MTT)法测定药物的细胞毒性及耐药细胞逆转倍数 ;荧光分光光度法测定细胞内药物浓度。结果 :无毒剂量的 β-榄香烯乳剂 (6 μg/ m l)能显著降低化疗药物 ADM对乳腺癌耐药细胞株 MCF- 7/ ADM细胞的 IC50 ,明显增加耐药细胞内药物浓度。结论 :初步研究表明β-榄香烯乳剂具有逆转 MCF- 7/ ADM细胞 MDR的作用。

Objective:To determine the non cytotoxic dose of β elemene and to investigate the reversal effect of β elemene on multidrug resistance in MCF 7/ADM cells.Methods:We determine the non cytotoxic dose of β elemene and the reversal fold by MTT assay.Then we set out the intracellular accumulation of ADM in MCF 7/ADM cell.Results:The non cytotoxic dose of β elemene(6μg/ml) can reduce the IC 50 of MCF 7/ADM cells and increase the intracelluar accumulation of ADM in MCF 7/ADM cells evidently.Conclusion:β elemene can reverse the drug resisance to ADM in MCF 7/ADM