烧伤猪肠道膜菌群的微生态失调研究

STUDYONDYSBIOSISOFINTESTINAL MEMBRANOUS FLORA IN BURNEDPIGS

本研究定量地分析了烧伤猪山莨菪碱治疗组和对照组的肠道粘膜及内容物中５类菌群，对内脏标本进行了细菌培养。其中治疗组猪的空肠膜菌群，肠杆菌的活菌计数（Ｌｇ１０￣（ｘ±ｓ））为５．６７±０．８５，肠球菌为２．６０±０．２３，双歧杆菌为５．２７±０．７１，类杆菌为３．９１±０．８３，乳杆菌为２．２０±０．１３；回肠膜菌群中５类菌活菌计数分别为５．８３±０．４２，２．５８±０．２１，５．６３±１．０２，４．０３±１．１１，２．５８±０．２１，５．６３±１．０２，４．０３±１．１１，２．５８±０．２；盲肠菌膜群依次为６．６２±１．２７，２．７１±０．４５，６．２３±１．５２，６．６７±１．２９，３．４３±１．２９；而盲肠内容物的５类菌数分别为８．０５±０．３１，３．５７±０．６５，７．７８±０．６２，６．９１±０．９６，５．４４±１．９８。内脏组织发生了细菌移位．对照组与治疗组都出现了微生态失调。结果显示山莨菪碱不能调整菌群失调症。

A quantitative analysis of species of indigenonus membranaceous and luminal bacterial flora, that is divided into checking test group and treating test group in burned pigs,was mede.In jejunal mucase of treating test groups the number of enterobacteria was 5. 67±0.85(CFU/g, Lg10),enterococci 2. 60 ±0. 23,bifidobacteria 5. 27±0.71,bacteroides 3. 91±0. 83 and Lactobacillus 2. 20±0.13,Whereas the counts in the ileal mucose were 5.83±0.02CFU/g,2.58±0. 2ICFU/g,5. 63±1. 02CFU/g,4. 03 ±1.11CFU/g,2. 58±0.2CFU/g,respectively, in the same order as above.Those in the cecal macase were, in the same order,6. 62±1.27CFU/g,2. 71±0. 45CFU/g,6. 23±1. 52CFU/g,6. 67±1.9CFU/g,3.43±1.29CFU/g,respctively,While those of the cecal contents were, in the order,8. 05 ±0.3ICFU/g,3.57±0.65CFU/g,7.78±0. 62CFU/g,6. 91±0. 96CFU/g,and 5. 44±1. 98CFU/g, respectively.The translocation of bacteria to the adjacent tissues was happened,the dysbiosis was appeared in burned pigs of control check test and treating test the dysbacteriosis was not treated by nisodamine.