脑啡肽-干扰素融合蛋白具有外周镇痛作用

A Peripheral Antinociceptive Effect of an Enkephalin-Met and Interferon α-m Fusion Protein

研究干扰素 -脑啡肽融合蛋白的外周镇痛作用和机制。对小鼠进行热损伤诱导 ,采用经典热板法测定小鼠后肢脚趾外周涂抹干扰素、脑啡肽融合蛋白的痛阈变化 ,并用阿片选择性拮抗剂纳曲酮、纳络酮及干扰素单抗进行阻断试验。与干扰素母体相比 ,融合蛋白具有较强的外周镇痛作用 ,这种作用可被纳络酮、干扰素单抗逆转或阻断。融合蛋白具有较强镇痛功能 ,可作为外用镇痛候选药物 ,其作用机理与干扰素受体、阿片

The peripheral antinociceptive effect of an enkephalin and interferon α-m fusion protein was invertigated by local application,the mechanisms of the fusion protein were assessed also by administering selective delta-,mu-opioid receptor antagonists,interferon monoclonal antibody in mice Topically administration of the fusion protein produced significant antinociceptive effects in mice The antinociceptive effect of the fusion protein did not result from the activation of delta-opioid receptors,since treatment of mice with a selective delta-opioid receptor antagonist,was ineffective on blocking the fusion protein induced antinociception The antinocieptive effect of the fusion protein was not only significantly antagonized by nalaxone,a selective mu-opioid receptor antagonist,but also blocked by the interferon antibody Furthermore,the fusion protein has peripheral antinociceptive effect and the mechanisms of the antinociception were mediated by the μ opioid receptor,but not δ opioid receptor,and were related with interferon receptor