615小鼠ES细胞集落的分离及初步鉴定

The Derivation and Examination of Embryonic Stem Cell Colonies in 615 Mouse

目的 分离和培养 6 15小鼠的ES细胞集落 ,为建系打下基础。方法 以PMEF为饲养层分离 6 15小鼠的ES细胞集落 ,进行无饲养层培养 ,并对其进行初步鉴定。结果 ES细胞集落的出现率和传代成功率为 2 2 6 %和 0 94 % ,其ALP染色阳性 ,具有稳定的二倍体核型 ,可自发分化为多种类型的细胞。结论 成功分离和培养了6

Objective To isolate ES cell colonies in 615 mouse. Method\ ES cell colonies in 615 mouse were isolated by PMEF feeder layer and the primary examination of these colonies without feeder layer was made. Results\ The formation rate of ES cell colonies and the rate of successful propagation were 22.6% and 0.94%,with positive ALP staining, stable karyotype and differentiation ability. Conclusion\ ES cell colonies in 615 mouse were isolated successfully.