摘要
目的 观察MMTV Wnt 1转基因小鼠的乳腺癌发病情况及病理学变化规律。方法 观察MMTV Wnt 1转基因小鼠肿瘤发生情况 ,并采用原位移植将瘤组织置于裸鼠皮下 ,通过组织病理学切片来观察MMTV Wnt 1阳性转基因小鼠和移植鼠的病理学变化。结果 MMTV Wnt 1转基因小鼠最早从 7周龄开始出现乳腺瘤 ,发瘤鼠剖检可见脾、肝有不同程度的肿大 ,其他器官无明显病变 ;病理组织学检查发现发瘤鼠各脏器有不同程度的病变 ,但未出现肿瘤转移。将瘤组织移植裸鼠后 ,肿瘤可在裸鼠皮下生长 ,移植肿瘤病理学形态与原发瘤一致 ,未出现转移。结论 实验结果验证MMTV Wnt 1转基因小鼠可稳定自发乳腺肿瘤 。
Objective To make pathological observation of MMTV Wnt 1 transgenic mice with mammary tumor.Methods The mammary tumor of MMTV Wnt 1 transgenic mice was observed and the tumor tissue was transfered to nude mouse in site to check the pathological changes .Results Tumorgenesis occurred in spleen and liver while no obvious lesion appeared in other organs. No metastasis occurred at the time mammary tumors were detected.Transplanted tumors were with the similar biological features of primar tumor.Conclusion The MMTV Wnt 1 transgenie mice conld be an animal model with mammary tumor.
出处
《中国实验动物学报》
CAS
CSCD
2002年第3期151-154,F004,共5页
Acta Laboratorium Animalis Scientia Sinica
