三单位保存的艾氏腹水癌细胞株及克隆细胞蛋白质表达

Protein Expression of Ehrlich Ascites Carcinoma Cell Strain and Cloned Cells Stored in Three Institutes

目的 比较三个单位保存的艾氏腹水癌 (EAC)细胞株及克隆细胞的蛋白质表达。方法 对北京市肿瘤研究所 (北京 )保存的EAC进行克隆培养 ,从中选出 5株克隆 ;对武汉大学保种中心 (武汉 )、山东省医学科学院药物研究所 (山东 )及北京保存的EAC细胞株及 5株克隆细胞的SDS PAGE电泳图谱及免疫组化蛋白质分布进行了对比。结果 武汉、山东及北京保存的EAC的电泳条带数分别为 2 2、2 5及 2 8条 ,而克隆细胞E2G8为 2 6条带。三单位EAC瘤细胞对 5种抗体做免疫组化染色 ,与 1株抗体反应的阳性细胞比例均较高 ,差异无显著性 (P >0 0 5 ) ,与另 4种抗体反应的阳性细胞率差异有显著性 ;5株克隆细胞对 10种抗体作免疫组化染色 ,其中克隆细胞E2G8、E2F4与 7种抗体反应阳性 ,E2C6与 8种抗体反应阳性 ,E1G5、E2B5与 6种抗体反应阳性。克隆细胞一旦与某种抗体反应阳性 ,阳性细胞的比例即在 85 %以上。

Objective To compare protein expression of EAC stored in different institutes and its cloned cells. Methods According to Fdler and Kripke's experimental evidence and theory, 5 cloned cells were isolated from EAC in Beijing Cancer Institute by single cell cloning technique. Proteins of EAC from Wuhan, Shandong,Beijing Cancer Institute and its cloned celles were compared by SDS PAGE and immunohistochemistry staining. Results The number of protein bands of EAC in Wuhan, Shandong and Beijing Cancer Institute was 22,25 and 28 respectively. The number of protein bands of cloned cell E2G8 was 26. The EAC cells in three institutes had different in the reaction to 5 kinds of antibodies and 5 cloned cells had different reactions to 10 kinds of antibodies. Cloned cell E2G8, E2F4 had positive reaction to 7 antibodies,while E2C6 to 8 antibodies and E1G5,E2B5 to 6 antibodies. And the positive reaction rates were all beyond 85%. Conclusion There were remarkable difference among EAC from different institutes and its cloned cells.