鱼类三种致病菌的粗脂多糖对异育银鲫的免疫原性

IMMUNOGENICITY OF CRUDE LIPOPOLYSACCHARIDE OF THREE PATHOGENIC BACTERIA OF FISH TO SILVER CRUCIAN CARP, CARESSIUS AURATUS GIBELIO

用温酚法从柱状嗜纤维菌、嗜水气单胞菌和鳗弧菌中提取的粗脂多糖 (LPS)作为免疫原 ,分别接种异育银鲫后 ,通过测定受免鱼的交叉凝集抗体效价 ,头肾和血液中吞噬细胞的吞噬活性和用A .hydrophila活菌攻毒的方法 ,探讨鱼类 3种致病菌的粗LPS对异育银鲫免疫原性的试验结果。结果表明 ,从 3种致病菌中提取的粗LPS对异育银鲫均具有较强的免疫原性 ,受免鱼的血清中存在对 3种致病菌的交叉凝集抗体 ;与对照鱼相比 ,头肾和血液中吞噬细胞的吞噬活性明显上升 ,受免鱼对A .hydrophila活菌攻毒均产生了较强的免疫保护力。说明在每种供试菌的粗LPS上都不同程度地存在 3种致病菌的共同保护性抗原。

The silver crucian carp (Caressius auratus gibelio )were immunized with crude lipopolysaccharide(LPS) extracted from Cytophaga columnaris?Aeromonas hydrophila and Vibrio anguillarum .The relative percent survival(RPS) of silver crucian carp were compared by examing the agglutinating antibody titers,cross agglutinating antibody titers of serum and the phagocytic activity of phagocytes in the blood and head kidney challenged with live A.hydrophila .The experimental results showed that the agglutinating antibody titers and cross agglutinating antibody titers of immunized silver crucian carp were higher than the control group,and the phagocytic activity of phagocytes in the blood and head kidney of silver crucian carp was significantly boosted and had a high level cross RPS.The crude LPS of three pathogenic bacteria were thought to have a common protective antigens to causing a cross RPS to silver crucian carp.