羟基喜树碱柔性脂质体冻干剂抗肿瘤药效学研究

Study on Antitumor Effects of Lyophilized HCPT Flexible Liposomes

目的研究羟基喜树碱柔性脂质体冻干剂体内外抗肿瘤作用。方法以市售羟基喜树碱注射液为对照,采用MTT法分别进行了羟基喜树碱柔性脂质体冻干剂对肝癌Hep G-2细胞、胃癌BGC-823细胞及结肠癌LOVO细胞体外抗肿瘤作用,并以人肝癌Hep G-2细胞建立了荷瘤裸鼠动物模型,考察了羟基喜树碱柔性脂质体冻干剂的体内抗肿瘤作用。结果羟基喜树碱柔性脂质体冻干剂对肝癌Hep G-2、胃癌BGC-823具有抗肿瘤作用且效果优于市售羟基喜树碱注射液,但对结肠癌LOVO细胞具有体外抗肿瘤作用较市售羟基喜树碱注射液要差,对裸鼠肝癌Hpe G-2移植瘤的生长有较好的抑制作用且作用强于阳性对照药市售羟基基喜树碱。结论柔性脂质体冻干剂后在一定程度上提高了羟基喜树碱的抗肿瘤作用。

OBJECTIVE To study the antitumor effects of lyophilized HCPT flexible liposomes in vitro and in vivo. METHOD Using the marketing HCPT injections as control, the in vitro antitumor effect of lyophilized HCPT flexible liposomes against Hep G-2, BGC-823 and LOVO cells were evaluated by MTT method. A Hep G-2 cell bearing nude mice model was established to investigate the in vivo antitumor effect of the lyophilized HCPT flexible liposomes. RESULT The antitumor effect of lyophilized HCPT flexible liposomes to Hep G-2 and BGC-823 cells was significantly higher than the marketing HCPT injections, while the effect to LOVO cells was lower than the marketing HCPT injections. The antitumor effect of lyophilized HCPT flexible liposomes to Hep G-2 cell bearing nude mice model was stronger than the marketing HCPT injections. CONCLUSION The lyophilized HCPT flexible liposomes enhanced the antitumor effect of HCPT.