人体不同解剖部位表皮基底层中CD34蛋白表达及意义

Analysis of Non-equilibrium Distribution of CD34 Molecules in Basal Layer of Epideris in Different Anatomical Parts of Human

目的:探讨人体不同解剖部位表皮基底层中CD34蛋白表达及意义。方法:采用组织学观察法、双免疫标记法、流式细胞术检测人包皮、头皮和躯干皮肤来源的组织标本中CD34蛋白表达。结果:CD34蛋白在人包皮和头皮的表皮基底层细胞中有表达,而在人躯干皮肤的表皮中无表达。Ⅰ型表位CD34蛋白的分布比Ⅱ、Ⅲ型表位CD34蛋白更加广泛。CD34阳性的基底层细胞同时也有B1整合素或p63的表达,并随着细胞传代而减少。不同年龄CD34Ⅰ型表位的阳性表达率均明显高于Ⅱ型和Ⅲ型(P<0.05),而不同年龄组的CD34阳性率各表位比较无显著性差异(P>0.05)。结论:CD34蛋白主要分布于包皮和头皮的表皮基底层细胞中,可用于包皮和头皮的表皮基底层细胞的分选。

Objective :To investigate the anatomic distribution of CD34 protein in different body parts of the basal layer of the epidermis.Methods:The expressions of CD34 protein was detected by histological observation.double immunogold 1 abeling,and flow cytometry was used to detect in the foreskin,scalp and trunk was more skin derived tissues.Results:CD34 protein was expressed in human foreskin and scalp epidermal basal layer widely cells,but not in the epidermis of skin trunk.The Ⅰ epitope of CD34 protein distributed than U, Ⅲ epitope CD34 protein.The CD34 positive cells also showed the expression of inte-grin beta l or p63,which decreased with cell passage.The positive expression of CD34 at Ⅰ epitope was much higher than that of Ⅱ type and Ⅲ type(P<0.05),while the positive rate of CD34 with the epitopes showed no significant difference between different age groups(P>0.05).Conclusion:The CD34 protein is mainly distributed in the basel layer of the epidermis of prepuce and scalp,and can be used sorting for epidermal basal cell of prepure and scalp.