紫杉素磁靶向脂质体的研制及其抗肿瘤作用的研究

Study on preparation of magnetoliposomes-entrapped taxol

目的 :制备紫杉素磁性脂质体 ,考察有关理化性质、在小鼠体内的分布及其抑瘤作用。方法 :用逆相蒸发法制备紫杉素磁性脂质体 ,对其包封率、粒径和 pH值等性质进行了研究 ,用高效液相色谱法测定了在兔体内的药动学参数 ,测定了其对小鼠EMT 6肿瘤的抑瘤率。结果 :紫杉素磁性脂质体包封率为 97.2 5 % ,平均粒径为 5 2 6nm ,pH值为6.2 0。结论 :制备得到的紫杉素磁性脂质体适宜于非胃肠道给药 ,且具有明显的抑瘤作用。

OBJECTIVE To study the preparation of paclitaxel entrapped magnetoliposomes (TAX MLP) and evaluate their quality. METHODS The magnetoliposomes were prepared by reverse phase evaporation method (REV).The morphology of TAX MLP, the entrapment efficiency and the pH were studied.RESULTS The mean size of magnetoliposomes was 577 nm and the extent of entrapment of the TAX MLP was 97.25% .The pH was 6.20 .CONCLUSIONS The technology of preparation of paclitaxel magnetoliposomes is practicable and successful.