里氏木霉与黑曲霉共同培养固态发酵生产饲用酶的研究

Feed Enzyme Production by Solid-State Fermentation through Co-culturing Aspergillus Nigervar and Tricheodrem Reesei

介绍了里氏木霉与黑曲霉共生用固态发酵工艺生产饲料用酶的方法。借助同时糖化与发酵的概念 ,在里氏木霉生长繁殖进入旺盛时期适时接入黑曲霉。而黑曲霉与里氏木霉共生 ,不仅有益于酶系的改善 ,而且黑曲霉的生长繁殖消耗了纤维素酶促水解所生成的葡萄糖等 ,有益于纤维素酶的合成。与此同时生成适量的酸性蛋白酶、果胶酶及糖化酶 ,提高了原料的利用价值。研究结果表明 ,影响酶活力的关键因素是培养基的水分及接种的时间 。

The method of producing feed enzyme by solid-state fermentation through co-culturing Asp. niger and T. reesei was studied. For concurrent saccharification and fermentation, the spores of Asp. niger were inoculated to the medium at proper time to be co-cultured with T. reesei which was vigorously growing then. Such symbiotic culturing was beneficial not only to the improvement of enzyme formulation but also to the increase of cellulase due to that glucose was consumed by Asp. niger . Meanwhile, the simultaneously produced acid-resistant proteinase, pectonase and glucosidase improved the utilization value of raw materials. It was found that the key factors affecting the activity of enzyme were the medium moisture content and inoculation time. The amount and type of cellulose were also very important.