C_(21)甾苷提取方法的研究

Studies on Extraction Methods of C_(21)Steroidal Glycosides

为建立萝摩科植物及相应的保健产品中C21甾苷的定量方法,本文首先进行各种提取方法的探讨。将用于强心苷薄层层析定量显色的2—去氧糖反应进行了改进,作为进行各种提取方法C21甾苷相对含量比较的方法。根据C21甾苷的结构,选用了11种萃取剂进行了提取实验,并探讨了原料粉碎度、提取时间等其它条件对提取效果的影响,最终确定了C21甾苷的提取方法。

In order to establish the quantitative determination method for C21 steroidal glycosides in Asclepiadaceae plants and homologous health food,the extraction methods of C21 steroidal glycosides were investigated fist in this paper.The reaction of 2-desoxy sugar for cardiac glycosides'quantitative determination by thin layer chromatogaphy was used as a relative quantitative determination method for the comparison of all kinds of extraction methods.Accorfing to the structure of C21 steroidal glycosides,11 kinds of extractes were selected to do extraction experiments.The effects of other factors Such as mesh size of the material and the extraction time were also studied. At last the extraction method of C21 steroidal glycosides was determined.