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人era的结构特点分析及其定点突变体的构建 被引量：2

Structural analysis of human era and construction of its site-directed mutants
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摘要 目的　构建最近克隆的人era基因的定点突变体。方法　利用数据库对era的结构特点进行分析 ,在此基础上用改良的重叠延伸法分别构建人EraN端和C端的定点突变体。结果　获得了分别针对人EraN端TGP结合结构域 (位于2 9 - 36氨基酸残基 )和C端具有RNA结合活性的KH结构域 (位于 2 97- 340氨基酸残基 )的定点突变体。 Objective To construct site directed mutants of human era.Methods The structural character of human era was analyzed by computer,era mutants were constructed by improved overlap extention method.Results Three site directed mutants were constructed successfully,one sited within GTP binding domain and the other two within KH domain.Conclusion These results may shed light on further functional study of human Era.
作者 纪宗玲 陈苏民 陈南春 吴元明 刘继中 路凡 张俊杰
机构地区 第四军医大学生物化学与子生物学教研室 第四军医大学西京医院全军骨科研究所
出处 《免疫学杂志》 CAS CSCD 北大核心 2002年第5期325-328,共4页 Immunological Journal
基金 国家自然科学基金 (39870 38030 0 0 0 0 91 ) 全军医药卫生科研基金 (98M1 0 8)资助项目
关键词 结构特点 定点突变体 构建 ERA基因 基因突变 human era improved overlap extention method site directed mutation
分类号 R392 [医药卫生—免疫学]
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参考文献6

  • 1[1]Ahnn J, March PE, Takiff HE, et al. A GTP-binding protein of Escherichia coli has homology to yeast RAS proteins[J].Proc Natl Acad Sci USA,1986,83(23):8 849-8 853.
  • 2[2]Zuber M, Hoover TA, Dertzbaugh MT,et al. Tularensis DNA clone complements E. coli defective for the production of era, an essential Ras-like GTP-binding protein[J]. Gene,1997,189(1):31-34.
  • 3[3]Chen SM, Takiff HE, Barber AM, et al. Expression and characterization of RNase Ⅲ and Era proteins. Products of the rnc operon of Escherichia coli[J]. J Biol Chem, 1990,265(5):2 888-2 895.
  • 4[4]Chen X, Court DL. Crystal structure of ERA: A GTPase-dependent cell cycle regulator containing an RNA binding motif[J]. Proc Natl Acad Sci USA,1999, 96(2):8 396-8 401.
  • 5[5]Britton RA, Powell BS, Dasgupta S,et al. Cell cycle arrest in Era GTPase mutants: a potential growth rate regulated cell cycle checkpoint in Escherichia coli[J]. Mol Microbiol, 1998,27(13):739-750.
  • 6[6]Britton RA, Chen SM, Wallis D, et al. Isolation and preli-minary characterization of the human and mouse homologues of the bacterial cell cycle gene era[J]. Genomics,2000,67(1):78-82.

同被引文献31

  • 1董轲,陈苏民,李云峰,吴元明,陈南春,张晓楠.不同剪切形式的小鼠Era对L-929细胞的影响[J].第四军医大学学报,2005,26(8):673-675. 被引量：2
  • 2Abu Sayed.Shin-ichi Matsuyama,Masayori Inouye.Era, an Essential Escherichia coli Small G-Protein,Binds to the 30S Ribosomal Subunit. Biochemical and Biophysical Research Communications,1999, 264:51 - 54.
  • 3Meier, T.I., Peery, R.B., McAllister, K.A. & Zhao, G.Era GTPase of Escherichia coli: binding to 16S rRNA and modulation of GTPase activity by RNA and carbohydrates. Microbiology,2000 146:1071 - 1083.
  • 4Zuber M, Hoover TA, Dertzbaugh MT, et al. A Francisella tularensis DNA clone complements Escherichia coli defective for the production of Era, an essential Ras-like GTP-binding protein [ J].Gene,1997, 189 (1) : 31-34.
  • 5Julie Q. Hangl and Genshi Zhao.Characterization of the 16S rRNA- and membrane-binding domains of Streptococcus pneumoniae Era GTPase. Eur. J. Biochem,2003,270:4164 - 4172.
  • 6Meier, T.I., Peery, R.B., Jaskunas, S.R. & Zhao, G.16S rRNA is bound to Era of Streptococcus pneumoniae. J. Bacteriol,1999,181:5242 - 5249.
  • 7Gollop N, March PE: A GTP-binding protein (ERA) has an essential role in growth rate and cell cycle control in Escherichia coli.J Bacteriol,1991,173:2265-2270.
  • 8Lin, Y.P., Sharer, J.D. & March, P.E. GTPase-dependent signaling in bacteria: characterization of a membrane-binding site for Era in Escherichia coli. J. Bacteriol, 1994 176:44 - 49.
  • 9Gollop, N. & March, P.E.Localization of the membranebinding sites of Era in Escherichia coli. Res. Microbiol,1991 142:301 - 307.
  • 10AkiyamaT, GohdaJ, Shibata S, et al. Mammalian homologue of E.coli Ras-like GTPase ( ERA ) is a possible apoptosis regulator with RNA binding activity [ J ] . Genes Cells,2001, 6 ( 11 ) : 987 - 1001.

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免疫学杂志
2002年 第5期

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