AML-M1相关TCR Vβ克隆性增殖T细胞的研究

Analysis of clonal expansion of TCR Vβ subfamily T cells associated with AML-M1

目的 了解急性髓细胞白血病M1亚型 (AML M1 )患者外周血TCRVβ亚家族T细胞的克隆性分布特点。 方法采用RT PCR扩增 9例M1患者外周血的单个核细胞的TCRVβ2 4个亚家族基因 ,分析其TCRVβ亚家族的利用情况。PCR产物进一步经基因扫描分析 ,了解其CDR3长度以判断T细胞的克隆性。结果 不同标本中可检测到 2～ 5个Vβ亚家族T细胞 ,以Vβ2、Vβ3、Vβ1 7和Vβ1 9为常见 ,并在Vβ2、Vβ3、Vβ5、Vβ8、Vβ9和Vβ1 9中发现克隆性生长T细胞。结论 M1病人外周血TCRVβ亚家族T细胞表达呈现明显的选择性和克隆性增殖情况 ,这可能与M1细胞相关抗原有关 ,且克隆性增殖Vβ亚家族分布以个体特异性为主。

Objective To investigate the distribution and clonality of TCR Vβ subfamily T cells in patients with M1 subtype of acute myelogenous leukemia (AML M1). Methods The CDR3 size of TCR Vβ 24 subfamily genes was amplified in peripheral blood mononuclear cells from 9 cases with AML M1 using RT PCR, to observe the usage of TCR Vβ repertoire, the PCR products were further analyzed by gene scan technique for detection of the CDR3 size, to evaluate clonality of TCR Vβ T cells.Results 2～5 Vβ subfamily T cells could be identified from different samples in M1 cases, predominantly in Vβ2,3,17 and 19. Oligoclonal (clonal expansion) T cells were identified in Vβ2, 3, 5, 8, 9 and 19 subfamilies.Conclusions The selected usage and clonal expansion of TCR Vβ subfamily T cells from peripheral blood could be found in patients with M1, it may be related to M1 associated antigen. Distribution of Vβ subfamily clonal expansion displays individual specificity.