成人急性B淋巴细胞白血病免疫表型特征研究

Immunophenotypic Features of Adult B-lymphoblastic Leukemia

目的 探讨干 /祖细胞标志CD3 4和髓系相关抗原在B -ALL中的表达特征。方法 采用一组系列相关单克隆抗体和流式细胞术对 10 8例成人急性B淋巴细胞白血病 (B -ALL)进行免疫表型分析。结果 10 8例B -ALL ,均表达CD10、CD19或CD2 2。CD3 4+前B淋巴细胞白血病在所有成人B -ALL中占 77.8%。My +B -ALL在所有成人B -ALL中占 3 2 .4%。髓系抗原表达在CD3 4+B -ALL与CD3 4-B -ALL两者之间差异显著 (P <0 .0 0 1)。CD3 4表达在My +B -ALL与My -B -ALL两者之间差异显著 (P <0 .0 0 2 )。结论 成人My +B -ALL起源早于My -B -ALL ,主要来源于造血干

Objective To explore the features of hematopoietic stem/progenitor cell marker CD34 and myeloid-associ ated antigen(My) expression in acute B-lymphoblastic leukemia(B-ALL) in adults. Methods Immunophenotyping was performed by a panel of lineage-associated monoclonal antibodies,CD34,immunofluorescence technique and flow cytometry in 108 patients with B-ALL.Results CD10,CD19 or CD22 were expressed in all 108 B-ALL,and CD34,My were expressed in 77.8% and 32.4% respectively of 108 B-ALL patients. My expression was significantly higher in CD34+ than in CD34- B-ALL ( P <0.001),and CD34 expression was higher in My+ than My- B-ALL ( P <0.002). Conclusion Adult My+B-ALL malignant transformation occurs earlier than My-B-ALL and is derived from hematopoietic stem/progenotor cells.