卡氏肺孢子虫在八种细胞系上的增殖情况

A comparative study on the growth of Pneumocystis carinii in 8 cell lines

目的 了解卡氏肺孢子虫在HepG 2 (人肝癌细胞 )等 8种细胞系的增殖状况 ,寻找适合卡氏肺孢子虫 (Pc)增殖的支持细胞系。方法 分别以 5× 10 5个虫体接种于 8种细胞系上。另采用HepG 2细胞 ,分别接种不同数量的Pc ,观察 7d ,比较Pc在不同细胞及不同接种数量的增殖情况。 结果 在HepG 2细胞上Pc滋养体可增殖 7倍左右 ,增殖高峰大多在第 4、5天。接种 5× 10 5个Pc ,包囊和滋养体均可较好增殖。结论 HepG 2细胞是较好的卡氏肺孢子虫体外培养支持细胞系 ,接种量以每孔 5× 10

Objective To identify the best cell line for Pneumocystis carinii(Pc) proliferation Methods Specimens containing 5×10 5 Pc cysts were inoculated onto 8 kinds of cell lines separately. At the same time, Pc were inoculated in different amounts on HepG-2 cell line to identify the most suitable number of Pc needed for Pc proliferation in vitro within seven days. Results On HepG-2 cell line, Pc increased by 7-fold in trophozoite forms with a peak increasing rate between 4～5 days with the optimal growth at the cyst number of 5×10 5 .Conclusions HepG-2 cell line is found to be the most suitable cell line for Pc culture and the best inoculation number of Pc is 5×10 5.