维生素A缺乏对大鼠红细胞膜流动性的影响

Affect of Vitamin A Deficiency on Erythrocyte Membrane Fluidity of Rats

目的 探讨维生素 A缺乏对大鼠红细胞膜流动性的影响。 方法 采用断乳期 2 1～ 2 3d Wistar大鼠 ,体重35～ 4 5 g,雌、雄各半 ,随机分为正常组及 VA缺乏组 ,分别饲喂正常饲料及无 VA的饲料 ,喂养 90 d后 ,腹主动脉取血 ,测定血清 VA含量 ,采用荧光偏振法 ,以 DPH为探针 ,测定红细胞膜流动性。 结果 正常组的雄、雌大鼠血清 VA水平为(0 .89± 0 .0 4 ) umol/ L ,而 VA缺乏组雄、雌性大鼠血清 VA水平分别降低到 (0 .14± 0 .0 5 ) umol/ L ,(0 .13± 0 .0 4 ) umol/ L ,两组比较 ,差异有极显著性 (t=4 0 .86 ,4 7.2 3,P<0 .0 1) ;维生素 A缺乏组雄、雌鼠红细胞膜的粘滞度高于正常组雄、雌鼠(t=3.2 0 ,2 .97,P<0 .0 1) 结论 维生素

Objective To study the affect of vitamin A deficiency on erythrocyte membrane fluidity of rats. Methods Wistar rats 21~23 days after brith were divided into 2 groups randomly. One group was fed normal forage as control,the other group was fed vitamin A deficiency forage.After 90 days,serum vitamin A was presented by fluorimetric method.The fluidity of erythrocyte membrane was determined with fluorescence polarization (p) mean microviscosity (η) by using DPH as a probe. Result The serum vitamin A of male and female rats fed normal forage was (0.89±0.04)umol/L .That fed vitamin A deficiency forage was (0.14±0.05)umol/L and (0.13±0.04)umol/L. It was significantly decreased(t=40.86,47.23, P<0.01). Erythrocyte membrane liquidity of the group with vitamin A deficiency was significantly decreased(t=3.20,2.97,P<0.01). Conclusion Vitamin A deficiency destroyed the stability of cell membrane.