自身免疫性甲状腺疾病HLA-DR抗原的免疫组化研究

IMMUNOHISTOLOGIC STUDY ON HLA-DR ANTIGEN EXPRESSION IN AUTOIMMUNE THYROID DISEASE

作者用碱性磷酸酶抗碱性磷酸酶(APAAP)免疫组化法研究19例桥本甲状腺炎(HT)。11例Graves病(GD)和4例非毒性甲状腺肿(NTG)的甲状腺滤泡细胞(TFC)HLA-DR抗原的异常表达。发现TFC DR抗原表达与自身抗体值无明确相关性,而与甲状腺中淋巴细胞浸润程度密切相关,与浸润淋巴细胞的DR抗原表达相关更密切。提示活化T细胞能促进TFC DR抗原的表达.在HT,TFCDR抗原的表达强于OD和NTG。

To further understand the correlation between aberrant HLA-DR expression and thyroid autoimmuhity, 40 thyroid specimens from thick-needle biopsy and surgery were analyzed by APAAP immunohistologic technique using HLA-DR monoclonal antibody. The expressions of HLA-DR antigen on thyroid follicular cells (TFC) was involved in almost every patient. The degree of DR expression was significantly higher in Hashimoto's thyroiditis (HT) than in Graves disease (GD) and nontoxic goiter (NTG), and there was no significant difference between GD and NTG. Above all, the degree of DR expression on TFC not only correlated markedly with the degree of lymphocytic infiltration in the thyroid tissue, but more so with the one of DR expression of the lymphocytes themselves; nevertheless, it did not correlate obvious- ly with the levels of circulating thyroglobulin antibody or thyroid microscmal antibody. It was suggested that the cyto- kines such as IFN-γ from activated T lymphocytes might induce the expression of HLA class II antigens on TFC.