用不提取血清刺激人甲状腺细胞cAMP的生成测定甲状腺刺激性抗体

DETERMINATION OF THYROID-STIMULATING ANTIBODIES WITH CYCLIC AMP ACCUMULATION INDUCED BY UNFRACTIONATED SERUM USING CULTURED HUMAN THYRIOD CELLS

用不提取的血清刺激培养的人甲状腺细胞cAMP的生成测定甲状腺刺激性抗体。结果显示,甲状腺刺激性抗体的阳性率在未治的Graves病患者为83.3％(25/30),抗甲状腺药物治疗的Graves病患者为43.8％(7/6),正常人为4％(1/25)。单纯性甲状腺肿和甲状腺腺瘤患者甲状腺刺激性抗体均为阴性。提示用本法检测Graves病患者的甲状腺刺激性抗体具有较高的敏感性和特异性。

Thyroid-stimulating antibodies were determined with cyclic AMP accumulation induced by unfractionaled serum using cultured human thyroid cells. Thyroid-stimulating antibodies were found in 83.3% (25/30) of patients with Graves' disease without treatment, 43.8% (7/16) of patients with Graves' disease during antithyroid treatment, 4% (1/25) of normal people and none of patients with simple goitre or thyroid adenoma. The results indicate that determination of thyroid- stimulating antibodies using this method is sensitive and characteristic in patients with Graves' disease.