摘要
石油污染土壤的生物修复受到很多条件的限制,特别是其中高效石油降解菌的获取。本文通过投加嗜盐菌和驯化的高效石油降解土著菌于石油污染土壤中,旨在强化石油污染土壤生物修复过程,获得石油的高效降解效果。研究结果表明:在土壤含油量高达19.51g/(kg干土),含盐量高达8.26g/(kg干土)中,投加嗜盐菌和土著菌的修复效果比不投加嗜盐菌的高出9.8%,表明了嗜盐菌和土著菌的共同作用加强了土壤的高效生物修复效果。根据PCR-DGGE的DNA指纹图谱可以看出土壤微生物菌群结构和优势种群经历了动态演替过程,不同修复时期的土壤中存在共同种属和特异种属,各个时期的生物多样性丰富,验证了石油污染土壤得到了高效修复,表明在石油污染土壤的生物修复过程中投加嗜盐菌和驯化的高效石油降解土著菌能明显改善修复效果,是一种可行的方法。
Many restrictions had limited the bioremediation of petroleumcontaminated soil, especially the obtaining of high oildegradation bacteria. In order to investigate if there was a high degradation, halophilic and indigenous bacteria were added into the bioremediation of petroleum-contaminated soil. The results showed that: in the soil containing a high oil concentration of 19.51 g/ (kg dry soil) and the salinity of 9.26 g/(kg dry soil) , the degradation was 9.8% higher in the soil with adjunction of halophilic and indigenous bacteria than only with the indigenous bacteria. According to the DGGE profile, dynamic changes of the bacteria structure and predominant bacteria was showed, the common bacteria and special bacteria could be detected in different phase of bioremediation, and the diversities of the bacteria communities were abundant, which all certified the high degradation of oil in the petroleumcontaminated soil. Hence, adding halophilic and indigenous bacteria to the process of bioremediation in the petroleum-contaminated soil would improve the oil degradation.
