海南岛糙海参性腺发育的组织学特征

Histological characteristics of gonadal development of Holothuria scabra from Hainan Island

对中国海南岛糙海参(Holothuria scabra)的性腺发育过程进行了组织学观察。结果表明,海南糙海参的性腺发育可分为恢复期(Ⅰ期)、增长期(Ⅱ期)、成熟期(Ⅲ期)、部分排放期(Ⅳ期)、排空期(Ⅴ期)共5个时期。1)精巢发育特征:Ⅰ期,生殖上皮出现精原细胞和精母细胞,生殖管壁最厚;Ⅱ期,生殖管上皮变薄,褶皱往管腔中延伸;Ⅲ期,成熟精子充满整个生殖管;Ⅳ期,生殖上皮恢复褶皱形态;Ⅴ期,生殖管中剩余少量残留精子。2)卵巢发育特征:Ⅰ期,在生殖上皮附近附着卵原细胞和卵黄发生前期卵母细胞;Ⅱ期,大量卵黄发生时期卵母细胞与卵原细胞及卵黄发生前期卵母细胞共存于生殖管中;Ⅲ期,卵黄发生时期卵母细胞充满生殖管;Ⅳ期,在生殖上皮附近重新出现卵黄发生前卵母细胞,生殖管腔中出现营养性吞噬细胞;Ⅴ期,生殖管腔残留少数卵细胞。

The characteristics of gonadal development of Holothuria scabra from Hainan Island were investigated by histological observation. Results show that gonadal development of H. scabra from Hainan Island were divided into five stages: recovery stage( Ⅰ),growing stage( Ⅱ),mature stage( Ⅲ),partly spawned stage( Ⅳ) and spent stage( Ⅴ). The characteristics of testis development are as follows: at StageⅠ,a few spermatogonias and spermatocytes started to grow along the germinal epithelium and the germ tube wall was the thickest. At Stage Ⅱ,the germinal epithelium became thinner and the longitudinal folds extended into the lumen. At Stage Ⅲ,lumens packed densely with mature spermatozoas,while at Stage Ⅳ,the germinal epithelium recovered into the original folding morphology. At Stage Ⅴ,a few of relic spermatozoa remained in the lumen. The characteristics of ovary development are as follows: at StageⅠ,oogonias and previtellogenic oocytes started to grow along the germinal epithelium. At Stage Ⅱ,central lumen contained numbers of vitellogenic oocytes,surrounded by oogonias and previtellogenic oocytes. At Stage Ⅲ,the lumen densely packed with vitellogenic oocytes. At Stage Ⅳ,the previtellogenic oocytes re-presented in germinal epithelium,and some nutritional phagocytes began to occur in lumen. At Stage Ⅴ,a few of relic vitellogenic oocytes were still in lumen.