新型重组人肿瘤坏死因子引起小鼠肝损伤

N,C-terminal mutant recombinant human tumor necrosis factor inducing hepatic injury in mice

目的 :研究新型重组人肿瘤坏死因子 (rhTNF NC)引起小鼠肝损伤的作用。方法 :给小鼠尾静脉注射rhTNF NC ,检测血清丙氨酸氨基转移酶 (ALT)和天门冬氨酸氨基转移酶 (AST)活性、肝脏指数 ,并用硫代巴比妥酸(TBA)测定肝组织中丙二醛 (MDA)含量 ,同时测定超氧化物歧化酶 (SOD)及谷胱甘肽过氧化物酶 (GSH Px)活性。结果 :给小鼠尾静脉注射rhTNF NC 8h后 ,血清ALT、AST活性显著升高 (P <0 0 1) ,肝脏指数和MDA含量也明显增加(P <0 0 5 ) ,而SOD活性下降 (P <0 0 5 ) ,但GSH Px活性未见明显改变 (P >0 0 5 )。结论 :rhTNF NC可引起小鼠脂质过氧化性肝损伤。

Objective:To investigate the effect of N,C-terminal mutant recombinant human tumor necrosis factor (rhTNF-NC) inducing hepatic injury in mice. Methods:Alanine aminotransferase (ALT), aspate aminotransferase (AST) activities in serum and the liver indexes were measured after injecting rhTNF-NC into the veins of the mice’s tails. Malondialdehyde (MDA) content, superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities in the liver tissues of the mice were measured with thiobarbituric acid (TBA). Results:Eight hours after rhTNF-NC was injected, the ALT and AST activities in serum increased significantly ( P &lt;0 01). The liver indexes and the MDA content also increased significantly( P &lt;0 05), while SOD activities decreased ( P &lt;0 05), but GSH-Px activities did not have any obvious change ( P &gt;0 05). Conclusion:rhTNF-NC can induce lipid peroxide hepatic injury in mice.