海水淹溺肺水肿兔血浆MDA和SOD的变化

Changes of malondialdehyde and superoxide dismutase in plasma in rabbits with pulmonary edema after seawater drowning

目的 :观察海水淹溺肺水肿 (PE SWD)兔血浆中丙二醛 (MDA)和超氧化物歧化酶 (SOD)变化规律 ,探讨上述两项指标发生变化的原因和机制。方法 :用核黄素光照法和硫代巴比妥酸法 ,对PE SWD兔动脉血浆MDA和SOD进行定量检测和动态观察。结果 :灌海水后 30min ,MDA和SOD较灌海水前均显著升高 ,尔后两者均逐渐下降 ,但仍远远高于灌海水前。结论 :PE SWD发生发展过程中 ,MDA的损害性作用使肺泡表面活性物质表面特性发生改变 ,影响了正常的呼吸功能 ,并掩盖了SOD的保护性作用。

Objective:To observe the changes of malondialdehyde (MDA) and superoxide dismutase (SOD) in plasma in rabbits with pulmonary edema after seawater drowning(PE-SWD) and to probe into the cause and mechanism of the changes of the above two indexes.Methods:The levels of MDA and SOD in the artery plasma in rabbits with PE-SWD were determined quantitatively and were observed dynamically with the riboflavin illumination and thiobarbituric acid.Results:The levels of MDA and SOD increased significantly 30 minutes after infusing seawater,then the two indexes decreased gradually,but they were still much higher than those before infusing seawater.Conclusion:With the development of PE-SWD, the alveolar surface-active-mass surface properties may be changed by the harmful effect of MAD, which may influence the normal respiratory function and cover the protective role of SOD.