反义核酸对放疗诱导前列腺癌细胞VEGF高表达的抑制作用

The Inhibitory Action of the Antisense Oligodeoxynucleotide to the Expression of Vascular Endothelial Growth Factor by Radiotherapy in a Prostate Cancer Cell Line

目的 :探讨放疗过程中病人血清血管内皮生长因子 (VEGF)升高的机制 ,以及VEGF反义硫代寡核苷酸(AS ODN)对前列腺癌细胞VEGF表达分泌的抑制作用。 方法 :动态观察前列腺癌病人放疗期间血清VEGF变化 ;体外照射培养的前列腺癌PC3M细胞 ,观察放疗对肿瘤细胞VEGF表达分泌的诱导作用及AS ODN的抑制效应。 结果 :前列腺癌病人血清VEGF浓度在放疗开始后逐渐升高 ,15d左右达到峰值后开始下降 ,到 6 0d时基本恢复到正常。前列腺癌细胞受X线照射后VEGF的分泌较对照组明显升高 ,AS ODN加照射组癌细胞VEGF分泌量较单纯照射组显著下降 (P <0 .0 1)。 结论 :放疗可刺激前列腺癌细胞中VEGF的表达分泌 ,VEGFAS

Objectives: To investigate mechanism for the increasing level of serum vascular endothelial growth factor(VEGF) in tumour patients during radiotherapy and the inhibitory action of the antisense oligodeoxynucleotide(AS ODN) to the expression of VEGF protein by radiotherapy in the prostate cancer cell line (PC3M). Methods: To observe the changes of serum VEGF in the prostate cancer patients during radiotherapy dynamically and the inhibitory action of the antisense oligodeoxynucleotide to the expression of VEGF by radiotherapy in PC3M. Results: The changes of serum VEGF in three patients receiving radiotherapy had been observed continuously. The levels of serum VEGF began to increase when the patients received radiotherapy and rised up to peak value after fifteen days,then declined to the range of pre radiotherapy. Irradiating the PC3M cells with X rays significantly increased the VEGF expression and secretion. The expression of VEGF protein in the group treated by VEGF AS ODNs and X ray irradiation decreased significantly than the group treated only by X ray irradiation. Conclusions: The induction of VEGF protein expression by X ray irradiation in tumor cells may result in the increasing of the VEGF in the prostate cancer patients during radiotherapy and the induction can be blocked by VEGF AS ODNs.